Flow Cytometry Protocol

Id :
99
Type:

Date :
2018/04/08
Country :
United States
City :
Shirley
Location :
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel :
16316197922
Contact :
Emily Dean
Note :
Intracellular flow cytometry can be used to analyze a variety of intracellular molecules including cytokines, inflammatory mediators, transcription factors, and phosphoproteins. It can provide rich information concerning cellular function and signaling responses. Different from cell surface markers staining, intracellular antigens detection requires cell fixation and permeabilization before staining. Typically, cells are fixed with formaldehyde to preserve the cellular morphology, and then permeabilized with detergent or alcohol.

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