Flow Cytometry Protocol

99

2018/04/08

United States

Shirley

45-1 Ramsey Road, Shirley, NY 11967, USA

https://www.creative-diagnostics.com/flow-cytometry-protocol-intracellular-staining.htm

https://www.creative-diagnostics.com/flow-cytometry-protocol-intracellular-staining.htm

[email protected]

16316197922

Emily Dean

Intracellular flow cytometry can be used to analyze a variety of intracellular molecules including cytokines, inflammatory mediators, transcription factors, and phosphoproteins. It can provide rich information concerning cellular function and signaling responses. Different from cell surface markers staining, intracellular antigens detection requires cell fixation and permeabilization before staining. Typically, cells are fixed with formaldehyde to preserve the cellular morphology, and then permeabilized with detergent or alcohol.